Recombinant Human ASF1A Protein (His&T7 Tag) | PKSH032549

Recombinant Human ASF1A Protein (His&T7 Tag) | PKSH032549 | Gentaur US, UK & Europe Disrtribition

Synonyms: Histone Chaperone ASF1A; Anti-Silencing Function Protein 1 Homolog A; hAsf1; hAsf1a; CCG1-Interacting Factor A; CIA; hCIA; ASF1A

Active Protein: N/A

Activity: Recombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Protein Construction: Recombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 25.4 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 1mM DTT, 150mM NaCl, pH 8.0.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Research Area: epigenetics and nuclear signal