Recombinant Human APE1/APE Protein (His Tag) | PKSH030851

Recombinant Human APE1/APE Protein (His Tag) | PKSH030851 | Gentaur US, UK & Europe Disrtribition

Synonyms: DNA-(Apurinic or Apyrimidinic Site) Lyase; APEX Nuclease; APEN; Apurinic-Apyrimidinic Endonuclease 1; AP Endonuclease 1; APE-1REF-1; Redox Factor-1; APEX1; APE; APE1; APEX; APX; HAP1; REF1

Active Protein: N/A

Activity: A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed; with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed; with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 37 kDa

Formulation: Lyophilized from sterile PBS, pH 7.5

Reconstitution: Please refer to the printed manual for detailed information.

Background: The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease; DNA 3' repair diesterase; 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously; the levels varied significantly; suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis; relatively high levels in the thymus; spleen; kidney and brain; and the lowest level in the liver in rats. However; the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain; especially in the hippocampal formation; to regulate some brain functions by redox-activation.

Research Area: Cardiovascular, epigenetics and nuclear signal