Recombinant Human ANPEP/CD13 Protein (603 Met/Ile, His Tag)(Active) | PKSH031912

Recombinant Human ANPEP/CD13 Protein (603 Met/Ile, His Tag)(Active) | PKSH031912 | Gentaur US, UK & Europe Disrtribition

Synonyms: APN;CD13;GP150;LAP1;P150;PEPN

Active Protein: Active protein

Activity: A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967，603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967，603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 104.2 kDa

Formulation: Lyophilized from sterile PBS, pH 7.3

Reconstitution: Please refer to the printed manual for detailed information.

Background: Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

Research Area: immunology, Stem cells