Description
Recombinant Human ALOX5AP/FLAP Protein (His Tag) | PKSH031059 | Gentaur US, UK & Europe Disrtribition
Synonyms: FLAP
Active Protein: N/A
Activity: A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2) (Met 1-Pro 161) was fused with a polyhistidine tag at the carboxy-terminus.
Protein Construction: A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2) (Met 1-Pro 161) was fused with a polyhistidine tag at the carboxy-terminus.
Fusion Tag: C-His
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 19 kDa
Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% gly, pH 7.4, 0.5% triton, 5mM DTT
Reconstitution: Please refer to the printed manual for detailed information.
Background: Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.
Research Area: Signal Transduction, Cardiovascular, immunology,
