Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active) | PKSH030690

Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active) | PKSH030690 | Gentaur US, UK & Europe Disrtribition

Synonyms: IAP

Active Protein: Active protein

Activity: A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503);without the pro peptide; was fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503);without the pro peptide; was fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 54 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: ALPI encodes for intestinal phosphatase alkaline; a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.

Research Area: Signal Transduction, Tags & Cell Markers, Cell biology, Cancer, immunology, metabolism,