Recombinant Human ALDOB/Aldolase B Protein (GST Tag) | PKSH030700

Recombinant Human ALDOB/Aldolase B Protein (GST Tag) | PKSH030700 | Gentaur US, UK & Europe Disrtribition

Synonyms: ALDB;ALDO2

Active Protein: N/A

Activity: A DNA sequence encoding the human ALDOB (P05062) (Ala 2-Tyr 364) was fused with the GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human ALDOB (P05062) (Ala 2-Tyr 364) was fused with the GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 66.5 kDa

Formulation: Lyophilized from sterile PBS, pH 7.5

Reconstitution: Please refer to the printed manual for detailed information.

Background: UBE2G1 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Research Area: Signal Transduction, Cancer, metabolism,