Recombinant Human AKT3 Protein (GST Tag) | PKSH031438

Recombinant Human AKT3 Protein (GST Tag) | PKSH031438 | Gentaur US, UK & Europe Disrtribition

Synonyms: MPPH;MPPH2;PKB-GAMMA;PKBG;PRKBG;RAC-gamma;RAC-PK-gamma;STK-2

Active Protein: N/A

Activity: A DNA sequence encoding the full length of human PKBG (NP_005456.1) (Met 1-Glu 479) was expressed with the GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the full length of human PKBG (NP_005456.1) (Met 1-Glu 479) was expressed with the GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 81 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Research Area: Signal Transduction, Neuroscience, Cancer, epigenetics and nuclear signal, metabolism,