Recombinant Human AIM2 Protein (GST Tag)(Active) | PKSH031042

Recombinant Human AIM2 Protein (GST Tag)(Active) | PKSH031042 | Gentaur US, UK & Europe Disrtribition

Synonyms: PYHIN4

Active Protein: Active protein

Activity: A DNA sequence encoding the human AIM2 (NP_004824.1) (Met 1-Thr 343) was fused with the GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human AIM2 (NP_004824.1) (Met 1-Thr 343) was fused with the GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 65.2 kDa

Formulation: Lyophilized from sterile 50mM Tris, 1M NaCl, 0.5mM PMSF, 5mM GSH, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: AIM2, Absent In Melanoma 2 is a member of the interferon-inducible HIN-200 protein family that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/oligosaccharide-binding domain, senses cytoplasmic DNA by means of its oligonucleotide/oligosaccharide-binding domain and interacts with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. In response to foreign cytoplasmic DNA, AIM2 forms an inflammasome, resulting in caspase activation in inflammatory cells. It had been pointed to a role of AIM2 function in both inflammation and cancer. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.

Research Area: Cancer, epigenetics and nuclear signal