Recombinant Human ACP1/LMW-PTP Protein (GST Tag)(Active) | PKSH031337

Recombinant Human ACP1/LMW-PTP Protein (GST Tag)(Active) | PKSH031337 | Gentaur US, UK & Europe Disrtribition

Synonyms: HAAP;Low Molecular Weight Phosphotyrosine Protein Phosphatase; LMW-PTP; LMW-PTPase; Adipocyte Acid Phosphatase; Low Molecular Weight Cytosolic Acid Phosphatase; Red Cell Acid Phosphatase 1; ACP1

Active Protein: Active protein

Activity: A DNA sequence encoding human ACP1 (AAI06012.1) (Met 1-His 158) was fused with the GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding human ACP1 (AAI06012.1) (Met 1-His 158) was fused with the GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 44.3 kDa

Formulation: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.

Research Area: N/A