Recombinant Human ACOX1/aox Protein (His Tag) | PKSH031175

Recombinant Human ACOX1/aox Protein (His Tag) | PKSH031175 | Gentaur US, UK & Europe Disrtribition

Synonyms: ACOX;PALMCOX;SCOX

Active Protein: N/A

Activity: A DNA sequence encoding the human ACOX1 (AAH08767.1) (Met 1-Leu 660) was expressed, with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human ACOX1 (AAH08767.1) (Met 1-Leu 660) was expressed, with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 76.7 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 20% gly, 3mM DTT

Reconstitution: Please refer to the printed manual for detailed information.

Background: Peroxisomal acyl-coenzyme A oxidase 1(ACOX1 or AOX) is the first enzyme of the fatty acid beta-oxidation pathway and belongs to the Acyl-CoA oxidase family. Human liver peroxisomes contain two acyl-CoA oxidases, namely, palmitoyl-CoA oxidase (ACOX1/AOX) and a branched chain acyl-CoA oxidase. The palmitoyl-CoA oxidase (ACOX1/AOX) oxidizes the CoA esters of straight chain fatty acids and prostaglandins and donates electrons directly to molecular oxygen, thereby producing H2O2. Human ACOX1/AOX is a protein of 661-amino acids, including the carboxyl-terminal sequence(Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. Human ACOX1/AOX, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse partly because of the Substrate utilization differences.

Research Area: N/A