Recombinant Human ACO1/irp1 Protein (His Tag) | PKSH031334

Recombinant Human ACO1/irp1 Protein (His Tag) | PKSH031334 | Gentaur US, UK & Europe Disrtribition

Synonyms: ACONS;HEL60;IREB1;IREBP;IREBP1;IRP1

Active Protein: N/A

Activity: A DNA sequence encoding the human ACO1 (P21399) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human ACO1 (P21399) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 101 kDa

Formulation: Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0, 10% glycerol, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase. 

Research Area: Signal Transduction, Cardiovascular, Cancer, epigenetics and nuclear signal, metabolism,