Description
Recombinant Human 14-3-3 sigma/YWHAS Protein | PKSH031400 | Gentaur US, UK & Europe Disrtribition
Synonyms: 14-3-3 Protein Sigma; Epithelial Cell Marker Protein 1; Stratifin; SFN; HME1
Active Protein: N/A
Activity: A DNA sequence encoding the human SFN (NP_006133.1) (Met 1-Ser 248) was expressed; with two additional amino acids (Gly & Pro) at the N-terminus.
Protein Construction: A DNA sequence encoding the human SFN (NP_006133.1) (Met 1-Ser 248) was expressed; with two additional amino acids (Gly & Pro) at the N-terminus.
Fusion Tag:
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 97 % as determined by reducing SDS-PAGE.
Endotoxin: Please contact us for more information.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 28 kDa
Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10mM GSH, 25% glycerol, pH 8.0
Reconstitution: Please refer to the printed manual for detailed information.
Background: 14-3-3 protein sigma (YWHAS); also known as stratifin (SFN) and epithelial cell marker protein 1; is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases; phosphatases; and transmembrane receptors; and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2; and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally; YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
Research Area: Signal Transduction, Neuroscience, Stem cells
