Description
Recombinant Human 14-3-3 beta/YWHAB Protein (GST Tag) | PKSH031394 | Gentaur US, UK & Europe Disrtribition
Synonyms: GW128;HEL-S-1;HS1;KCIP-1;YWHAA
Active Protein: N/A
Activity: A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was fused with the GST tag at the N-terminus.
Protein Construction: A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was fused with the GST tag at the N-terminus.
Fusion Tag: N-GST
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 96 % as determined by reducing SDS-PAGE.
Endotoxin: Please contact us for more information.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 54.9 kDa
Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5
Reconstitution: Please refer to the printed manual for detailed information.
Background: 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.
Research Area: Signal Transduction, Cell biology, Neuroscience, Stem cells
