Description
Recombinant HCV (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag) | PKSV030179 | Gentaur US, UK & Europe Disrtribition
Synonyms: NS3 Protein, HCV
Active Protein: N/A
Activity: A DNA sequence encoding the Hepatitis C virus (HCV)(serotype 1a, isolate H77) NS3 (NP_803144.1) (Thr1356-Thr1459) was expressed with a GST tag at the N-terminus
Protein Construction: A DNA sequence encoding the Hepatitis C virus (HCV)(serotype 1a, isolate H77) NS3 (NP_803144.1) (Thr1356-Thr1459) was expressed with a GST tag at the N-terminus
Fusion Tag: N-GST
Species: HCV
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: Please contact us for more information.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 37.9kDa.
Formulation: Lyophilized from sterile 50 mM Tris, 500 mM NaCl.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Background: HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS.
Research Area: Virus related Protein
