Description
RBFOX3 Polyclonal Antibody | E-AB-70267 | Gentaur US, UK & Europe Disrtribition
Reactivity: Mouse, Rat
Host: Rabbit
Isotype: IgG
Application: WB, IHC
Research Area: Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Tags and Cell Markers
Background: Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Lead Time: 7~10 days
Concentration: 1 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: KLH conjugated Synthetic peptide corresponding to NeuN
Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:300-1:800
Conjugation: Unconjugated
Calculated Molecular Weight: 46-48 kDa
Observed Molecular Weight: 46-48 kDa
Clonality: Polyclonal
