Description
Rat SHH(Sonic Hedgehog Protein) ELISA Kit | E-EL-R0484 | Gentaur US, UK & Europe Disrtribition
Target Species: Rat
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
Sensitivitys: 0.1ng/mL
Detection Range: 0.16~10ng/mL
Target Name: SHH
Target Synonyms: HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, Sonic hedgehog
Tested Sample: Serum, plasma and other biological fluids
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SHH. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat SHH. You can calculate the concentration of Rat SHH in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat SHH in samples. No significant cross-reactivity or interference between Rat SHH and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Rat SHH concentrations in Serum, plasma and other biological fluids.
Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Developmental biology, Stem cells
Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
