Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit | E-EL-R0520

Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit | E-EL-R0520 | Gentaur US, UK & Europe Disrtribition

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivitys: 0.19ng/mL

Detection Range: 0.31~20ng/mL

Target Name: NOS2/iNOS

Target Synonyms: NOS2A, HEP-NOS, I-NOS

Tested Sample: Serum, plasma and other biological fluids

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in Serum, plasma and other biological fluids.

Research Area: Cancer, Metabolism, Neuroscience

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.