Rat ICAM-1/CD54(Intercellular Adhesion Molecule 1) ELISA Kit | E-EL-R2850

(No reviews yet) Write a Review
SKU:
575-E-EL-R2850
Weight:
1.00 KGS
€762.00
Frequently bought together:

Description

Rat ICAM-1/CD54(Intercellular Adhesion Molecule 1) ELISA Kit | E-EL-R2850 | Gentaur US, UK & Europe Disrtribition

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivitys: 0.19ng/mL

Detection Range: 0.31~20ng/mL

Target Name: ICAM-1/CD54

Target Synonyms: BB2, P3.58

Tested Sample: Serum, plasma and other biological fluids

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1/CD54. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat ICAM-1/CD54. You can calculate the concentration of Rat ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Rat ICAM-1/CD54 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat ICAM-1/CD54 concentrations in Serum, plasma and other biological fluids.

Research Area: Cancer, Cardiovascular, Immunology, Stem cells

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

View AllClose