Rat Hepatic Stellate Cells | CP-R041

Rat Hepatic Stellate Cells | CP-R041 | Gentaur US, UK & Europe Disrtribition

Abbreviation: R-HSC

Organism: Rat

Tissue Type: Digestive system

Tissue: Liver tissue

Cell Type: Sternzellen

Growth Proprieties: Adherent

Medium: CM-R041

Ratio: 1:2-1:3

Renwal: Every 2-3 days

Background: Rat hepatic stellate cells were isolated from liver tissue. The liver is an organ in the body whose main function is metabolism, and it plays a role in deoxidation, storage of glycogen, and synthesis of secreted proteins. The liver also makes bile in the digestive system. The liver is the largest organ in the internal organs of the body. It is located in the abdomen in the body, under the right diaphragm, at the front of the gallbladder and in front of the right kidney and above the stomach. The liver is the largest digestive gland in the body's digestive system. It is a reddish-brown V-shaped organ. The liver is the main organ for urea synthesis and an important organ for metabolism. The position and morphological structure of the liver in the body: the liver is located in the upper right abdomen, hidden under the right side of the diaphragm and the deep surface of the ribs. Most of the liver is covered by the costal arch, which is only exposed in the upper abdomen area and between the right costal arch and directly touches the front of the abdomen. The upper part of the liver is connected with the diaphragm and the anterior abdominal wall. Hepatic stellate cells (HSC), also known as liver fat storage cells, vitamin A storage cells, pericytes, Ito cells, etc., are the main source of liver extracellular matrix. HSC can be further transformed into myofibroblast-like cells after activation. Various factors that can cause liver fibrosis all use HSC as the final target cell. Under normal circumstances, HSC is in a static state. When the liver is damaged by inflammation or mechanical stimulation, the phenotype of HSC changes from static to active. Activated HSC can participate in the formation of liver fibrosis and the reconstruction of liver structure through proliferation and secretion of extracellular matrix. This process is also the central link of liver fibrosis. Hepatic stellate cells are distributed in the Disse space of the liver. They are the main source of synthesis and secretion of extracellular matrix, and play the most important role in the occurrence of liver fibrosis. Hepatic stellate cells are mesenchymal cells unique to the liver, accounting for about 8%-13% of the total liver cells. As the main cell group for the liver to synthesize extracellular matrix, hepatic stellate cells can not only secrete extracellular matrix components such as proteoglycans and glycoproteins, synthesize a certain amount of collagenase to maintain the normal basement membrane structure, and it can also participate in the microcirculation regulation of the liver sinusoids through the contraction of its protrusions. In addition, hepatic stellate cells can promote hepatocyte proliferation and liver regeneration by synthesizing hepatocyte growth factor, insulin-like growth factor, epidermal growth factor, etc. The rat hepatic stellate cells produced by our company are prepared by mixed enzyme perfusion digestion, low-speed centrifugation, and density gradient centrifugation. The total number of cells is about 5×10^5 cells/vial. The cells are identified by Desmin/alpha-SMA immunofluorescence, and the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, etc.

Delivery: 5 weeks