Description
Rat Dermal Fibroblast | CP-R086 | Gentaur US, UK & Europe Disrtribition
Abbreviation: R-DF
Organism: Rat
Tissue Type: Integumentary system
Tissue: Dermal tissue
Cell Type: Fibroblast
Growth Proprieties: Adherent
Medium: CM-R086
Ratio: 1:2-1:3
Renwal: Every 2-3 days
Background: Rat dermal fibroblasts are isolated from dermal tissue. The dermis, located in the deep layer of the epidermis, is connected to the subcutaneous tissue downwards. The collagen fibers and elastic fibers of the dermal connective tissue are intertwined with each other and are embedded in the matrix. There are a variety of connective tissue cells and a large number of collagen fibers and elastic fibers, which make the skin elastic and tough. It is composed of two layers-the papillary layer and the reticular layer. The structure of the dermis is collagen, elastic fibers and matrix. Fibroblasts are the main cellular components of loose connective tissue, which are derived from the mesenchyme in the embryonic period. Fibroblasts are large, with clear outlines, mostly protruding spindle-shaped or star-shaped flat structures, with regular oval nuclei and large and obvious nucleoli. Fibroblasts have vigorous functions and activities. The cytoplasm is weakly basophilic and has obvious protein synthesis and secretion activities. Under certain conditions, it can achieve mutual transformation with fibroblasts. Fibroblasts are very important for the repair of different degrees of cell degeneration, necrosis and tissue defects Effect. The newly isolated dermal fibroblasts are round and have good refractive index. They are suspended in the culture medium. After 30 minutes, the cells adhere to the flask, and some of them begin to protrude pseudopods, which appear as small protrusions. After 6 hours, the cells are almost completely attached. It stretches into a fusiform with clear nuclei, relatively uniform distribution, scattered growth, and does not aggregate into clusters. The cells grow rapidly and reach confluence within 5-7 days. The cells are tightly arranged, and some overlap and grow, flat and large. The cytoplasm is transparent, the nucleus is large, elliptical, and light in color. The cells reach confluence and connect to each other into a network. The cells are distributed in a protruding spindle or star-shaped flat. The main functions of dermal fibroblasts under physiological conditions include: constructing and maintaining the normal morphology of tissues, synthesizing and releasing extracellular matrix, and timely gathering and repairing damaged tissues after tissue damage. The rat dermal fibroblasts produced by our company are prepared by neutral protease digestion and then trypsin-collagenase mixed digestion combined with differential adhesion method, the total number of cells is about 5×10^5 cells/vial. The cells are identified by Vimentin immunofluorescence, and the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi, etc.
Delivery: 4 weeks
