Description
Rat CK-18/KRT18(Cytokeratin 18) ELISA Kit | E-EL-R1004 | Gentaur US, UK & Europe Disrtribition
Target Species: Rat
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
Sensitivitys: 0.1ng/mL
Detection Range: 0.16~10ng/mL
Target Name: CK-18/KRT18
Target Synonyms: CYK18, K18
Tested Sample: Serum, plasma and other biological fluids
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CK-18/KRT18. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CK-18/KRT18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CK-18/KRT18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat CK-18/KRT18. You can calculate the concentration of Rat CK-18/KRT18 in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat CK-18/KRT18 in samples. No significant cross-reactivity or interference between Rat CK-18/KRT18 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Rat CK-18/KRT18 concentrations in Serum, plasma and other biological fluids.
Research Area: Cancer, Signal transduction, Tags and Cell Markers
Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
