Raji Cell Line | CL-0189

Raji Cell Line | CL-0189 | Gentaur US, UK & Europe Disrtribition

Media: CM-0189

Organism: Homo sapiens, Human

Application: Transfection.

Background: These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus(EBV) viral DNA sequences via PCR.

Age: 11 years

Tissue: Lymphoblast

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 2

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days