Description
Raji Cell Line | CL-0189 | Gentaur US, UK & Europe Disrtribition
Media: CM-0189
Organism: Homo sapiens, Human
Application: Transfection.
Background: These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus(EBV) viral DNA sequences via PCR.
Age: 11 years
Tissue: Lymphoblast
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 2
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
