RAD17 Polyclonal Antibody | E-AB-60923

RAD17 Polyclonal Antibody | E-AB-60923 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human

Host: Rabbit

Isotype: IgG

Application: WB, IF

Research Area: Epigenetics and Nuclear Signaling

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant fusion protein of human RAD17 (NP_002864.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Purification: Affinity purification

Dilution: WB 1:500-1:2000 IF 1:10-1:100

Conjugation: Unconjugated

Calculated Molecular Weight: 57 kDa/66 kDa/75 kDa/77 kDa

Observed Molecular Weight: 77 kDa

Clonality: N/A