Description
Rabbit Pulmonary Aorta Smooth Muscle Cells | CP-Rb009 | Gentaur US, UK & Europe Disrtribition
Abbreviation: Rb-PASMC
Organism: Rabbit
Tissue Type: Respiratory system
Tissue: Pulmonary aorta tissue
Cell Type: Myocyte
Growth Proprieties: Adherent
Medium: CM-Rb009
Ratio: 1:2-1:3
Renwal: Every 2-3 days
Background: Rabbit pulmonary aorta smooth muscle cells were isolated from the pulmonary aorta tissue. The pulmonary aorta is also called the pulmonary trunk. In air-breathing vertebrates, the arteries that direct venous blood from the heart to the lungs. The aorta pulmonary artery originates from the right ventricle and runs diagonally to the upper left and rear before the aorta. It is divided into the left and right pulmonary arteries under the aortic arch and enters the lungs through the hilum. The trunk of the pulmonary artery is a stubby trunk located in the pericardium. It starts from the right ventricle and runs diagonally from the front of the ascending aorta to the upper left, back and upper, to the left and right pulmonary arteries below the aortic arch. The left pulmonary artery is short, running across the front of the left main bronchus, dividing into two branches into the upper and lower lobes of the left lung. The right pulmonary artery is long and thick. It runs transversely to the right after passing through the ascending aorta and superior vena cava, and divides into three branches into the upper, middle and lower lobes of the right lung at the right hilum. Pulmonary arterial smooth muscle cells are one of the important structural cells of pulmonary blood vessels and play an important role in regulating the contraction and diastolic function of pulmonary blood vessels. After 3 days of primary isolation and culture of pulmonary aortic smooth muscle cells, the cells can be seen to adhere to the flask, and the cell morphology varies in size, fusiform, irregular, triangular or fan-shaped, and the nucleus is oval and centered. The cells reach confluence after 2 weeks, most of the cells stretch out in a long fusiform shape, with abundant cytoplasm and branch-like protrusions. The cells are arranged in a single layer or partially overlapped in multiple layers growth, ups and downs. When the cell density is low, cells are often intertwined into a network; when the density is high, they are arranged in a whirlpool or fence shape. After passage, the cells grow faster and can reach confluence in 4-6 days and maintain the above-mentioned morphological characteristics and growth characteristics. The abnormality of pulmonary aorta smooth muscle cells is an important pathological feature of the occurrence and development of pulmonary hypertension, and its apoptosis and proliferation imbalance are the key to pulmonary vascular remodeling. Studies have shown that both acute and chronic hypoxia can lead to pulmonary hypertension, namely hypoxic pulmonary hypertension. It is inferred that hypoxia may participate in the occurrence and development of hypoxic pulmonary hypertension by promoting the proliferation of pulmonary aortic smooth muscle cells. The main functions of pulmonary arterial smooth muscle cells: 1. The cell surface expression mediators (ICAM-1 and VCAM-1) participate in the inflammatory response of the vessel wall. 2. They are the target cells of most important arterial diseases. Pulmonary arterial smooth muscle cells and the main pathophysiological changes: 1. Smooth muscle hyperplasia easily causes pulmonary hypertension; 2. Congenital pulmonary artery stenosis; 3. Pulmonary embolism. The rabbit pulmonary aortic smooth muscle cells produced by our company are prepared by the trypsin-collagenase combined digestion method and the differential adhesion method. The total number of cells is about 5×10^5 cells/vial. The cells are identified by alpha-SMA Immunofluorescence, the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Delivery: 5 weeks
