Description
Rabbit Bladder Fibroblast | CP-Rb019 | Gentaur US, UK & Europe Disrtribition
Abbreviation: Rb-BF
Organism: Rabbit
Tissue Type: Urinary system
Tissue: Bladder tissue
Cell Type: Fibroblast
Growth Proprieties: Adherent
Medium: CM-Rb019
Ratio: 1:2-1:3
Renwal: Every 2-3 days
Background: Rabbit bladder fibroblasts are isolated from bladder tissue. The bladder is a urine storage organ. In mammals, it is a sac-shaped structure composed of smooth muscle, located in the pelvis, and its rear opening is connected to the urethra. There is a sphincter at the junction of the bladder and the urethra , which can control the discharge of urine. The bladder wall is composed of three layers of tissue, from the inside to the outside are the mucosal layer, the muscular layer and the adventitia. The muscular layer is composed of smooth muscle fibers, called detrusor.The contraction of the detrusor can increase the pressure in the bladder and press the urine out of the urethra. At the junction of the bladder and the urethra, there is a thicker ring muscle to form the internal urethral sphincter. Contraction of the sphincter can close the urethral opening and prevent urine from leaking from the bladder. The bladder wall is divided into three layers: the serosal layer (cellular fat tissue), the muscle layer (detrusor, bladder deltoid muscle) and the mucosal layer (very thin layer of transitional epithelial tissue). Fibroblasts are the main cellular components of loose connective tissue, which are derived from the mesenchyme in the embryonic period. Fibroblasts are large, with clear outlines, mostly protruding spindle-shaped or star-shaped flat structures, with regular oval nuclei and large and obvious nucleoli. Fibroblasts have vigorous functions and activities. The cytoplasm is weakly basophilic and has obvious protein synthesis and secretion activities. Under certain conditions, it can achieve mutual transformation with fibroblasts. Fibroblasts are very important for the repair of different degrees of cell degeneration, necrosis and tissue defects Effect. The newly isolated bladder fibroblasts are round and have good refractive index. They are suspended in the culture medium. After 30 minutes, the cells adhere to the flask, and some of them begin to protrude pseudopods, which appear as small protrusions. After 6 hours, the cells are almost completely attached. It stretches into a fusiform with clear nuclei, relatively uniform distribution, scattered growth, and does not aggregate into clusters. The cells grow rapidly and reach confluence within 5-7 days. The cells are tightly arranged, and some overlap and grow, flat and large. The cytoplasm is transparent, the nucleus is large, elliptical, and light in color. The cells reach confluence and connect to each other into a network. The cells are distributed in a protruding spindle or star-shaped flat. The growth factor secreted by bladder fibroblasts is a multifunctional mitogen. Studies have shown that it is involved in the formation of malignant tumors. In recent years, the role of basic fibroblast growth factor in the pathogenesis of bladder cancer, its relationship with biological behavior, and its therapeutic application have been paid attention. The rabbit bladder fibroblasts produced by our company are prepared by trypsin-collagenase mixed digestion method combined with differential adhesion method, the total number of cells is about 5×10^5 cells/vial. The cells are identified by Vimentin immunofluorescence, and the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi, etc.
Delivery: 5 weeks
