Description
QuicKey Human TF(Transferrin) ELISA Kit | E-TSEL-H0001 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 0.13ng/mL
Detection Range: 0.14~100ng/mL
Target Name: TF
Target Synonyms: N/A
Tested Sample: Serum, Plasma, Urine
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human TF. Samples (orStandards) and biotinylated detection antibody specific for Human TF are addedto the micro ELISA plate wells. Human TF would combine with the specificantibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessively to each micro plate well and incubated. Free components are washedaway. The substrate solution is added to each well. Only those wells thatcontain Human TF, biotinylated detection antibody and Avidin-HRP conjugate willappear blue in color. The enzyme-substrate reaction is terminated by theaddition of stop solution and the color turns yellow. The optical density (OD)is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD valueis proportional to the concentration of Human TF. You can calculate theconcentration of Human TF in the samples by comparing the OD of the samples tothe standard curve.
Specificity: This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human TF concentrations in Serum, Plasma, Urine .
Research Area: Cancer, Cardiovascular
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
