Description
QuicKey Human IgM(Immunoglobulin M) ELISA Kit | E-TSEL-H0010 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 1.02ng/mL
Detection Range: 7.81~500ng/mL
Target Name: IgM
Target Synonyms: N/A
Tested Sample: Serum, Plasma, Urine, Saliva
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgM. Samples (or Standards) and biotinylated detection antibody specific for Human IgM are added to the micro ELISA plate wells. Human IgM would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgM. You can calculate the concentration of Human IgM in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human IgM in samples. No significant cross-reactivity or interference between Human IgM and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human IgM concentrations in Serum, Plasma, Urine, Saliva.
Research Area: Cancer, Immunology
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
