Description
QuicKey Human Cys-C(Cystatin C) ELISA Kit | E-TSEL-H0008 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 0.04ng/mL
Detection Range: 0.31~20ng/mL
Target Name: Cys-C
Target Synonyms: N/A
Tested Sample: Serum, Plasma, Urine, Saliva
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) and biotinylated detection antibody specific for Human Cys-C are added to the micro ELISA plate wells. Human Cys-C would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. You can calculate the concentration of Human Cys-C in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human Cys-C in samples. No significant cross-reactivity or interference between Human Cys-C and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human Cys-C concentrations in Serum, Plasma, Urine, Saliva.
Research Area: Cancer, Cell Biology, Cardiovascular, Stem cells, Tags and Cell Markers
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
