Description
QuicKey Human CRP(C-Reactive Protein) ELISA Kit | E-TSEL-H0007 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 0.23ng/mL
Detection Range: 0.39~25ng/mL
Target Name: CRP
Target Synonyms: N/A
Tested Sample: Serum and plasma
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and biotinylated detection antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in Serum and plasma.
Research Area: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
