Description
QuicKey Human ADP/Acrp30(Adiponectin) ELISA Kit | E-TSEL-H0020 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 0.18ng/mL
Detection Range: 0.39~25ng/mL
Target Name: ADP/Acrp30
Target Synonyms: GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1
Tested Sample: Serum and plasma
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Samples (or Standards) and biotinylated detection antibody specific for Human ADP/Acrp30 are added to the micro ELISA plate wells. Human ADP/Acrp30 would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human ADP/Acrp30. You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human ADP/Acrp30 in samples. No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human ADP/Acrp30 concentrations in Serum and plasma.
Research Area: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
