Psi2 DAP cell line | CL-0416

Psi2 DAP cell line | CL-0416 | Gentaur US, UK & Europe Disrtribition

Media: CM-0416

Organism: Mus musculus, Mouse

Application: N/A

Background: The cell line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP). This cell line can produce a vector encoding the human placental alkaline phosphatase gene and the neomycin resistance gene, which can infect mouse cells. Infected cells express alkaline phosphatase that can be detected histochemically and thus being traced in vivo. Helper virus may be produced by this cell line.

Age: embryo

Tissue: Normal Embryo

Morphology: Fibroblast-Like

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days