POLR2A Antibody / RNA polymerase II subunit RPB1 | V7452IHC-7ML

POLR2A Antibody / RNA polymerase II subunit RPB1 | V7452IHC-7ML | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Format: Purified

Clone: CTD 4H8

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human, Mouse, Rat

Application: IHC-P

Buffer: N/A

Limitation: This POLR2A antibody is available for research use only.

Purity: Protein G affinity chromatography

Description: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Immunogen: The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.

Storage: Store the POLR2A antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).