Description
POLR2A Antibody / RNA polymerase II subunit RPB1 / Phosphorylated Serine 5 | V7525IHC-7ML | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Format: Purified
Clone: CTD 8A7
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: IHC-P
Buffer: N/A
Limitation: This phospho-RNA polymerase II antibody is available for research use only.
Purity: PEG precipitation
Description: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
Immunogen: The immunogen for this phospho-RNA polymerase II antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
Storage: Store the phospho-RNA polymerase II antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
