Description
POLR2A Antibody (pS2) | RQ4505 | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: Antibody in PBS with 0.02% sodium azide and 50% glycerol
Format: Purified
Clone: AH-16
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: Human
Application: WB
Application Details: Western blot: 1:500-1:2000
Application Note: Optimal dilution of the phospho-POLR2A antibody should be determined by the researcher.
Purity: Protein A affinity
Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA- dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. .
Immunogen: The amino acids surrounding phosphorylated Serine at position 2 were used as the immunogen for the phospho-POLR2A antibody.
Storage: After reconstitution, the phospho-POLR2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.
Localization: Nucleus
