PMEL17 / Melanoma gp100 Antibody Cocktail | V2857SAF-100UG

PMEL17 / Melanoma gp100 Antibody Cocktail | V2857SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: HMB45 + PMEL/783

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human

Application: IHC-P, WB

Buffer: N/A

Limitation: This gp100 antibody cocktail is available for research use only.

Purity: Protein G affinity chromatography

Description: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This mAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This mAb also stains Angiomyolipoma (PEComa).

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa.

Immunogen: Extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant human protein (PMEL/783) were used as the immunogen for the gp100 antibody cocktail.

Storage: Store the gp100 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).