PPM

 Plant Preservation Mixture (PPMTM) is a robust broad-spectrum biocide formulated for use in plant tissue culture. PPMTM targets bacteria and fungi in plant tissue culture growth media as well as contaminated tissue. It affects key enzymes in the Krebs cycle and in the Electron Transport Chain. Depending on the dose and the level of contamination, PPM is a biocidal component in plant culture medium. In addition, it may also function as a biostatic compound as a preventative measure. When diluted with plant growth media it is effective as a microbiocide (i.e. bactericide and fungicide) against non-human health pathogenic organisms component of liquid or semi-solid plant culture media. PPMTM is effective for most seed baring plants - angiosperm, as well as gymnosperm,- however, it is not recommended for use in ferns, mosses, algae and aquatic plants. Optimization may be required to maximize potency. While PPM is an excellent tool in the prevention and elimination of culture contamination it is not a substitute for asceptic laboratory techniques and appropriate air handling systems are recommended

PPM™ most definitely will facilitate the work in any plant tissue laboratory and should significantly increase technician and laboratory productivity. However, conditions in each lab may vary which could have a bearing on the effectiveness of PPM™. It is advisable that staff follow the above guidelines initially and adjust parameters accordingly. When used as recommended: • PPM™ is effective against airborne contamination, waterborne contamination and contamination introduced from human contact. • If used correctly, PPM™ will eliminate endogenous contamination from explants. • At recommended doses (0.5 - 2ml/l ), PPM™ does not impair in vitro seed germination, callus proliferation, callus regeneration, and axillary or adventitious buds' induction.