Phospho-SMAD3 (Ser425) Monoclonal Antibody | E-AB-51043

Phospho-SMAD3 (Ser425) Monoclonal Antibody | E-AB-51043 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse, Rat

Host: Mouse

Isotype: IgG

Application: IHC-p

Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Stem Cells

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic Peptide of Phospho-Smad3 (Ser425)

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification: Protein A purification

Dilution: IHC 1:50-300

Conjugation: Unconjugated

Calculated Molecular Weight: N/A

Observed Molecular Weight: N/A

Clonality: Monoclonal