Phospho-PI 3 kinase p85 alpha /gamma (Tyr467/199) Polyclonal Antibody | E-AB-20966

Phospho-PI 3 kinase p85 alpha /gamma (Tyr467/199) Polyclonal Antibody | E-AB-20966 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Isotype: IgG

Application: WB, IHC-p, IF, ELISA

Research Area: Cancer, Immunology, Signal Transduction

Background: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the phosphorylation site of Tyr467/199

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Purification: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000

Conjugation: Unconjugated

Calculated Molecular Weight: 54+83 kDa

Observed Molecular Weight: 55+85 kDa

Clonality: Polyclonal