Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody | E-AB-21121

Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody | E-AB-21121 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Isotype: IgG

Application: WB, IHC-p, IF, ELISA

Research Area: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction

Background: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Purification: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000

Conjugation: Unconjugated

Calculated Molecular Weight: 62 kDa

Observed Molecular Weight: 63 kDa

Clonality: Polyclonal