Description
Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody | E-AB-21121 | Gentaur US, UK & Europe Disrtribition
Reactivity: Human, Mouse, Rat, Monkey
Host: Rabbit
Isotype: IgG
Application: WB, IHC-p, IF, ELISA
Research Area: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction
Background: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.
Lead Time: 7~10 days
Concentration: 1 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172
Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000
Conjugation: Unconjugated
Calculated Molecular Weight: 62 kDa
Observed Molecular Weight: 63 kDa
Clonality: Polyclonal
