PD-L1 [Biotinylated]: B7-1 Inhibitor Screening ELISA Kit | EP-134-96tests

PD-L1 [Biotinylated]: B7-1 Inhibitor Screening ELISA Kit | EP-134| Gentaur Distribution US, UK & Europe

Programmed death molecule 1 and its ligand (PD-1/PD-L1) are a pair of negative immune costimulatory molecules. Under normal circumstances, the combination of PD-L1 on tissue cell surface and PD-1 on lymphocyte surface can inhibit lymphocyte function and induce apoptosis of activated lymphocyte, thus playing an autoimmune tolerance role. However, pD-L1 is also expressed on the surface of various tumor cells. Pd-l1 expressed by tumor cells can bind to PD-1 on the surface of corresponding lymphocytes, inhibit the function of lymphocytes and the release of cytokines, and induce apoptosis of lymphocytes, leading to immune escape of tumor cells. Therefore, blocking the PD-1/PD-L1 pathway can enhance lymphocyte activity to achieve the effect of tumor immunotherapy.

Source: Programmed death molecule 1 and its ligand (PD-1/PD-L1) are a pair of negative immune costimulatory molecules. Under normal circumstances, the combination of PD-L1 on tissue cell surface and PD-1 on lymphocyte surface can inhibit lymphocyte function and induce apoptosis of activated lymphocyte, thus playing an autoimmune tolerance role. However, pD-L1 is also expressed on the surface of various tumor cells. Pd-l1 expressed by tumor cells can bind to PD-1 on the surface of corresponding lymphocytes, inhibit the function of lymphocytes and the release of cytokines, and induce apoptosis of lymphocytes, leading to immune escape of tumor cells. Therefore, blocking the PD-1/PD-L1 pathway can enhance lymphocyte activity to achieve the effect of tumor immunotherapy.

Molecule: PDN/AL1.

Format: Solid.

Species: Human.

Application: This kit is developed for screening for inhibitors of human PD-L1 binding to human B7-1.

Kit Component: This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-L1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-L1 to immobilized human B7-1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated PD-L1 protein, a human B7-1 protein, an anti-PD-L1 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

Stability: The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Storage Conditions: 2-8℃.

Shipping Conditions: RT