PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit | 72026

PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit | 72026 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Assay Kit

Application: This kit is useful for screening for inhibitors of PD-L1 binding to B7-1.

Background: There have been a number of studies defining a role for PD-L1 in the regulation of immune tolerance and cancer immunity through binding to T cell-expressed PD-1. Interestingly, PD-L1 has also been shown to interact with B7-1, also known as CD80. Like PD- 1:PD-L1 interaction, B7-1:PD-L1 signaling also leads to inhibition of T cell activation, proliferation, and cytokine production, suggesting that this pathway may also serve as a target in cancer immunotherapy and inflammatory diseases.

Description: The PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the PD-L1:B7-1 interaction. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, B7-1-biotin is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Product Type: Assay Kit

Shippement Condition: -80°C (dry ice)