PD-1:PD-L2 Cell-Based Inhibitor Screening Assay Kit | 79378

PD-1:PD-L2 Cell-Based Inhibitor Screening Assay Kit | 79378 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Assay Kit

Application: • Screen for activators or inhibitors of PD-1 signaling in a cellular context • Characterize the biological activity of PD-1 and its interactions with ligands

Background: Programmed Cell Death Protein 1 (PD-1) is a receptor which is expressed on activated T-cells. Binding of PD-1 to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1: PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance.

Description: The PD-1/PD-L2 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L2 interaction. The assay consists of two main components: • Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing Human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment. • Expression vectors for TCR activator and Human PD-L2: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L2 and an engineered cell surface T cell receptor (TCR) activator.

Product Type: Assay Kit

Shippement Condition: -80°C