PD-1 [Biotinylated]: PD-L2 Inhibitor Screening ELISA Kit | EP-120-96tests

PD-1 [Biotinylated]: PD-L2 Inhibitor Screening ELISA Kit | EP-120| Gentaur Distribution US, UK & Europe

PD-L2, also known as CD273 or B7-DC, is a member of the B7 family protein with PD-L1, and it is the second important ligand that can bind pD-1 after PD-L1. The human PD-L2 (B7-H2, CD273) gene is also located on chromosome 9p24, encoding type I transmembrane protein with 247 amino acids. The homology of PD-L1 and PD-L2 was 40%. The affinity between PD-L2 and PD-1 is 2-6 times that of PD-L1. Compared with PD-L1, the expression of PD-L2 is relatively limited, and pD-L2 is induced to express on the surface of dendritic cells, macrophages, bone marrow derived mast cells and other cells.

Source: PD-L2, also known as CD273 or B7-DC, is a member of the B7 family protein with PD-L1, and it is the second important ligand that can bind pD-1 after PD-L1. The human PD-L2 (B7-H2, CD273) gene is also located on chromosome 9p24, encoding type I transmembrane protein with 247 amino acids. The homology of PD-L1 and PD-L2 was 40%. The affinity between PD-L2 and PD-1 is 2-6 times that of PD-L1. Compared with PD-L1, the expression of PD-L2 is relatively limited, and pD-L2 is induced to express on the surface of dendritic cells, macrophages, bone marrow derived mast cells and other cells.

Molecule: PDN/A1.

Format: Solid.

Species: Human.

Application: This kit is developed for screening for inhibitors of human PD1 binding to human PD-L2.

Kit Component: This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-1 to immobilized human PD-L2 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinlated PD-1 protein, a human PD-L2 protein, an anti-PD-1 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

Stability: The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Storage Conditions: 2-8℃.

Shipping Conditions: RT