Description
PCNA Antibody | F54492-0.2ML | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azide
Format: Purified
Clone: 1655CT506.10.26
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: WB, FACS, IHC-P
Buffer: N/A
Limitation: This PCNA antibody is available for research use only.
Purity: Protein G affinity
Description: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Immunogen: Recombinant human protein was used as the immunogen for the PCNA antibody.
Storage: Aliquot the PCNA antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
