Description
PC 61 5.3 [PC 61; PC 61.5.3] Cell Line | CL-0663 | Gentaur US, UK & Europe Disrtribition
Media: CM-0663
Organism: Mus musculus, Mouse; Rattus norvegicus, Rat
Application: N/A
Background: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Age: N/A
Tissue: Spleen
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+10%FBS+1%P/S(PB180120)
Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 5 x 105 cells/mL.
Ratio: 1:2 to 1:4
Renewal: Add fresh medium every 2 to 4 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days