Description
PBP Antibody | R30803 | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format: Antigen affinity purified
Clone: N/A
Host Animal: Rabbit
Clonality: Polyclonal (rabbit origin)
Species Reactivity: Mouse, Rat
Application: WB, IHC-P
Buffer: N/A
Limitation: This PBP antibody is available for research use only.
Purity: Antigen affinity
Description: Phosphatidylethanolamine-binding protein inhibits the phosphorylation and activation of MEK by RAF1. PBP coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. Overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PBP expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). Depletion of the protein decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.
Immunogen: An amino acid sequence from the N-terminus of mouse PBP (QEVDEPPQHALRVDY) was used as the immunogen for this PBP antibody (100% rat homology).
Storage: After reconstitution, the PBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.
