PARP2 Chemiluminescent Assay Kit | 80552-2

PARP2 Chemiluminescent Assay Kit | 80552-2 | Gentaur US, UK & Europe Disrtribition

Category: PARP/Assay Kit

Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput (HTS) applications.

Background: PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.

Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. *NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Product Type: Assay Kit

Shippement Condition: -80°C