Description
PARP12 Chemiluminescent Assay Kit | 78504 | Gentaur US, UK & Europe Disrtribition
Category: PARP/Assay Kit
Application: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and high throughput applications.
Background: N/A
Description: The PARP12 Chemiluminescent Assay Kit is designed to measure PARP12 activity for screening and profiling applications. PARP12 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP12 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP12 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP12 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Product Type: Assay Kit
Shippement Condition: -80°C
