Description
PA-1 Cell Line | CL-0411 | Gentaur US, UK & Europe Disrtribition
Media: CM-0411
Organism: Homo sapiens, Human
Application: N/A
Background: The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine. The embryonic antigen PCC4 is expressed, but F9 is not detectable.
Age: 12 years
Tissue: Ovary, derived from metastatic site: ascites
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~36 hours
Biosafty: 1
Medium: MEM (PM150410)+10% FBS+1% P/S (PB180120)
Subculturing: N/A
Ratio: 1:3 to 1:4
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+33% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10 (7) cells.
Duration: N/A
Lead Time: 3-5 days