Description
p65 Antibody NF-kB | R30293 | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format: Antigen affinity purified
Clone: N/A
Host Animal: Rabbit
Clonality: Polyclonal (rabbit origin)
Species Reactivity: Human, Mouse, Rat
Application: WB
Buffer: N/A
Limitation: This p65 antibody is available for research use only.
Purity: Antigen affinity
Description: The p65 (RELA) heterodimer is the most abundant form of NF-KB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A(PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B through 2 sites, an N-terminal domain that interacts with the C-terminal region of the unphosphorylated protein, and a second domain that only interacts with p65 phosphorylated on serine-276.
Immunogen: An amino acid sequence from the N-terminus of human NF-kB p65 (AISQRIQTNNNPFQVP) was used as the immunogen for this p65 antibody (100% homologous in human and rat).
Storage: After reconstitution, the p65 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.
